RNA-seq Workshop April 25th

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Beaulieu, Vickie

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Apr 22, 2013, 12:10:17 PM4/22/13

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**RNA-seq Workshop April 25th**

The Harvard School of Public Health Bioinformatics Core (HBC) will be offering a one-day introductory workshop on RNA-sequencing this **April 25th, 9am-5pm** at the Countway Library, Room L2-025 (Electronic Classroom).

Space is limited; REGISTER NOW if you would like to attend at http://rna-seq-catalyst-april25-2013.eventbrite.com/.

No prior programming experience or command line training is required.

RNA-seq is replacing microarrays as a new tool to study transcriptome changes through massively parallel "Next Generation" sequencing (NGS). The ability to read cDNA sequences directly makes it possible to study non-model organisms as well as model organisms with unprecedented sensitivity and reproducibility. The workshop will cover best practices for quality control, read alignment, and expression analysis using RNA-seq data.

# Who should attend?

Harvard affiliated Researchers who:

• are generating, or hope to generate RNA-seq data

• want to use a simple, graphical interface to analyze their results

• want to analyze their results in a reproducible fashion

# Format and Schedule

There are two sessions.

* Introduction to Galaxy (9am-11am): For those who have no experience using Galaxy. Recommended.

* RNA-seq using Galaxy (11am - 5pm, includes a break for lunch* and a short break in the afternoon): Introduces basic concepts and illustrates an end-to-end data analysis workflow through live tutorials using the Galaxy analytical framework. At the end of this activity, participants will be able to set up their own Galaxy servers using Amazon’s EC2 environment to analyze their data, without using the command line.

# Requirements

While biological and medical knowledge are helpful, students do not need to have prior programming or “command line” experience to participate in the course.

For those unfamiliar with the Galaxy platform, we will start the workshop with an optional introductory Galaxy tutorial, covering such topics as data import, filtering, combining, conversion and how to perform actions on genomic regions. After a short break we will spend the rest of our time applying this knowledge to RNA-seq.

Note that this introductory Galaxy session will be identical to the session preceding our previous Galaxy-based training workshops. Those who have attended these workshops or have other prior Galaxy experience can skip the first session and join us at 11am, though all are welcome to attend.

9am-5pm, Thursday April 25th, 2013

Countway Library, Room L2-025

Longwood Campus

10 Shattuck Street

Boston, MA 02115

* Lunch will not be provided, but there are a number of cafeterias and restaurants in the immediate vicinity.

Vickie S. Beaulieu

Program Coordinator

Harvard School of Public Health

Department of Biostatistics

655 Huntington Avenue

Building II, Room 402