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viable but non-culturable staphylococci in biofilms from central venous catheters negative on standard microbiological assays
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asfyso
May 19, 2012, 3:10:46 AM5/19/12
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Clin Microbiol Infect. 2012 Apr 16. doi: 10.1111/j.
1469-0691.2012.03893.x. [Epub ahead of print]
Detection of viable but non-culturable staphylococci in biofilms from
central venous catheters negative on standard microbiological assays.
Zandri G, Pasquaroli S, Vignaroli C, Talevi S, Manso E, Donelli G,
Biavasco F.
Source
Department of Life and Environmental Sciences, Polytechnic University
of Marche, Ancona Biochemical and Microbiology Laboratory, AO
'Ospedali Riuniti', Ancona Microbial Biofilm Laboratory, IRCCS
Fondazione Santa Lucia, Rome, Italy.
Abstract
Clin Microbiol Infect ABSTRACT: Viable bacteria were sought in 44 Maki-
negative biofilms from central venous catheters (CVCs) using
epifluorescence microscopy after live/dead staining. Thirty (77%)
samples contained viable but non-culturable (VBNC) cells; the majority
were positive on real-time PCR specific for Staphylococcus epidermidis
(one also for Staphylococcus aureus). Viable cells were significantly
(p <0.01) associated with CVCs from febrile patients, three of whom
showed S. epidermidis-positive blood cultures, suggesting that CVC-
associated biofilms can be reservoirs for staphylococci in the VBNC
state. The possible role of VBNC staphylococci in persistent
infections related to medical devices requires further investigation.
1469-0691.2012.03893.x. [Epub ahead of print]
Detection of viable but non-culturable staphylococci in biofilms from
central venous catheters negative on standard microbiological assays.
Zandri G, Pasquaroli S, Vignaroli C, Talevi S, Manso E, Donelli G,
Biavasco F.
Source
Department of Life and Environmental Sciences, Polytechnic University
of Marche, Ancona Biochemical and Microbiology Laboratory, AO
'Ospedali Riuniti', Ancona Microbial Biofilm Laboratory, IRCCS
Fondazione Santa Lucia, Rome, Italy.
Abstract
Clin Microbiol Infect ABSTRACT: Viable bacteria were sought in 44 Maki-
negative biofilms from central venous catheters (CVCs) using
epifluorescence microscopy after live/dead staining. Thirty (77%)
samples contained viable but non-culturable (VBNC) cells; the majority
were positive on real-time PCR specific for Staphylococcus epidermidis
(one also for Staphylococcus aureus). Viable cells were significantly
(p <0.01) associated with CVCs from febrile patients, three of whom
showed S. epidermidis-positive blood cultures, suggesting that CVC-
associated biofilms can be reservoirs for staphylococci in the VBNC
state. The possible role of VBNC staphylococci in persistent
infections related to medical devices requires further investigation.
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