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Optical coherence tomography for biofilm detection in chronic rhinosinusitis with nasal polyposis
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asfyso
May 19, 2012, 3:20:15 AM5/19/12
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Eur Arch Otorhinolaryngol. 2012 May 13. [Epub ahead of print]
Optical coherence tomography for biofilm detection in chronic
rhinosinusitis with nasal polyposis.
Tóth L, Vajas A, Csomor P, Berta A, Sziklai I, Karosi T.
Source
Department of Otolaryngology and Head and Neck Surgery, Medical and
Health Science Center, University of Debrecen, Nagyerdei Krt. 98.,
4032, Debrecen, Hungary.
Abstract
Chronic rhinosinusitis with nasal polyposis (CRSwNP) is a
multifactorial disease that seems to be associated with the presence
of microbial biofilms and corresponding subepithelial inflammatory
reactions. Optical coherence tomography (OCT) might be applied to
detect bacterial and fungal biofilms in patients with CRSwNP. A total
of 27 patients with CRSwNP undergoing endoscopic sinus surgery (ESS)
were analyzed. The negative control group consisted of six patients
undergoing septoplasty for nasal obstruction without CRSwNP. The nasal
polyps and inferior turbinate mucosa specimens applied as negative
controls were processed to OCT analysis and H.E. and Gram staining.
Biofilm was detected in 22 of 27 patients (81.5 %) with CRSwNP and in
none of six negative controls. In our series, OCT scan showed an
obvious association with the findings of H.E. and Gram staining and
was allocated to be a good predictor of biofilm existence. On OCT
images, biofilms were displayed as distinct superficial layers with
high optical density. It was found that microscopic architecture of
biofilms was strongly associated with the integrity of nasal mucosa
and to the cellular pattern of subepithelial inflammatory reaction.
This study confirmed the presence of microbial biofilms in patients
with CRSwNP according to OCT scans and histological analysis. Since
biofilms may affect the severity and recurrence rate of CRS treated by
ESS they should be detected preoperatively. In conclusion, single
application of OCT analysis or combination with conventional
histological protocols provides a robust and reliable method for the
detection of bacterial and fungal biofilms in CRSwNP. Level of
evidence 3b, individual case-control study.
Optical coherence tomography for biofilm detection in chronic
rhinosinusitis with nasal polyposis.
Tóth L, Vajas A, Csomor P, Berta A, Sziklai I, Karosi T.
Source
Department of Otolaryngology and Head and Neck Surgery, Medical and
Health Science Center, University of Debrecen, Nagyerdei Krt. 98.,
4032, Debrecen, Hungary.
Abstract
Chronic rhinosinusitis with nasal polyposis (CRSwNP) is a
multifactorial disease that seems to be associated with the presence
of microbial biofilms and corresponding subepithelial inflammatory
reactions. Optical coherence tomography (OCT) might be applied to
detect bacterial and fungal biofilms in patients with CRSwNP. A total
of 27 patients with CRSwNP undergoing endoscopic sinus surgery (ESS)
were analyzed. The negative control group consisted of six patients
undergoing septoplasty for nasal obstruction without CRSwNP. The nasal
polyps and inferior turbinate mucosa specimens applied as negative
controls were processed to OCT analysis and H.E. and Gram staining.
Biofilm was detected in 22 of 27 patients (81.5 %) with CRSwNP and in
none of six negative controls. In our series, OCT scan showed an
obvious association with the findings of H.E. and Gram staining and
was allocated to be a good predictor of biofilm existence. On OCT
images, biofilms were displayed as distinct superficial layers with
high optical density. It was found that microscopic architecture of
biofilms was strongly associated with the integrity of nasal mucosa
and to the cellular pattern of subepithelial inflammatory reaction.
This study confirmed the presence of microbial biofilms in patients
with CRSwNP according to OCT scans and histological analysis. Since
biofilms may affect the severity and recurrence rate of CRS treated by
ESS they should be detected preoperatively. In conclusion, single
application of OCT analysis or combination with conventional
histological protocols provides a robust and reliable method for the
detection of bacterial and fungal biofilms in CRSwNP. Level of
evidence 3b, individual case-control study.
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