Am J Clin Nutr. 2000 Dec;72(6):1488-94.
Contribution of dietary protein to sulfide production in the large
intestine: an in vitro and a controlled feeding study in humans.
Magee EA, Richardson CJ, Hughes R, Cummings JH.
Dunn Clinical Nutrition Centre, Hills Road, Cambridge, United Kingdom.
e.m...@dundee.ac.uk
BACKGROUND: Hydrogen sulfide is a luminally acting, bacterially derived
cell poison that has been implicated in ulcerative colitis. Sulfide
generation in the colon is probably driven by dietary components such as
sulfur-containing amino acids (SAAs) and inorganic sulfur (eg, sulfite).
OBJECTIVE: We assessed the contribution of SAAs from meat to sulfide
production by intestinal bacteria with use of both a model culture system in
vitro and an in vivo human feeding study. DESIGN: Five healthy men were
housed in a metabolic suite and fed a sequence of 5 diets for 10 d each.
Meat intake ranged from 0 g/d with a vegetarian diet to 600 g/d with a
high-meat diet. Fecal sulfide and urinary sulfate were measured in samples
collected on days 9 and 10 of each diet period. Additionally, 5 or 10 g
bovine serum albumin or casein/L was added to batch cultures inoculated with
feces from 4 healthy volunteers. Concentrations of sulfide, ammonia, and
Lowry-reactive substances were measured over 48 h.
RESULTS: Mean (+/-SEM) fecal sulfide concentrations ranged from 0.22 +/-
0.02 mmol/kg with the 0-g/d diet to 3.38 +/- 0.31 mmol/kg with the 600-g/d
diet and were significantly related to meat intake (P: < 0.001). Sulfide
formation in fecal batch cultures supplemented with both bovine serum
albumin and casein correlated with protein digestion, as measured by the
disappearance of Lowry-reactive substances and the appearance of ammonia.
CONCLUSION: Dietary protein from MEAT is an important substrate for
sulfide generation by bacteria in the human large intestine.
PMID: 11101476
The article does not mention the diet of the people involved. Somehow
I do not think it is vegetarian, but only pursuing the original
research will clear this up.