Question related to making track hub

[Li, Aiguo (NIH/NCI) [E]]

Dear Sir/Madam,

I am writing to ask assistance in making track hub.  I just created the hub.txt, genomes.txt and trackDb.txt files, debugged them using hubCheck and found no error.  I was able to load the http link to ucsc site without error.  However when adding mapping database http://genome.ucsc.edu/cgi-bin/hgTracks?db=hg19&hubUrl= in front of my http url, I got the error message: ERROR: No Content-Length: returned in header for http://genome.ucsc.edu/cgi-bin/hgTracks?db=hg19&hubUrl=http://helix.nih.gov/~NOB2/827hub/hub.txt, can't proceed,

I have checked my bigwig file accessibility from custom track and it works fine.  I checked the bigwig file format and noticed it does not have header (see below), but I am not sure this could be the issue or not. chr1 0 914078 -0.39102792739868164

chr1 914078 3628156 -0.028905002400279045

chr1 3628156 3647104 -0.43936997652053833

chr1 3647104 5295691 0.05502462387084961

chr1 5295691 5634053 0.05502462387084961

Any suggestions will be appreciated!

Aiguo (Anna) Li

Sr. Bioinformatician

Building 37, rm 1142

NOB/NCI/NIH

301-435-1454 (o)

[Hiram Clawson]

Good Morning Aiguo:

When I enter the example URL you provided:
http://genome.ucsc.edu/cgi-bin/hgTracks?db=hg19&hubUrl=...your.../hub.txt

I don't find any error in the hub. The data displays and the hub functions.
Is this the URL you are using to provide access to your hub data ?

You are missing the descriptionUrl E10747_827.html file from your hub.txt reference.

I do note that some of your wiggle data has 'nan' for a value instead of
an actual value. Plus, you appear to have too many significant digits
in your values. They are not illegal, but they seem meaningless, I doubt
any experiment has that much resolution.

--Hiram

On 12/2/15 8:19 AM, Li, Aiguo (NIH/NCI) [E] wrote:
> Dear Sir/Madam,
>

> I am writing to ask assistance in making track hub. I just created the hub.txt, genomes.txt and trackDb.txt files, debugged them using hubCheck and found no error. I was able to load the http link to ucsc site without error. However when adding mapping database http://genome.ucsc.edu/cgi-bin/hgTracks?db=hg19&hubUrl= in front of my http url, I got the error message: ERROR: No Content-Length: returned in header for http://genome.ucsc.edu/cgi-bin/hgTracks?db=hg19&hubUrl=http://helix.nih.gov/.../hub/hub.txt, can't proceed,