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Well, now I am especially confused. As of ~ 3:00 EDT, I started getting the error message below. I have changed no settings in any of my programs or config files. Is something happening with the browser?
mustOpenFd: Can't open phastCons46way.wib to read
getaddrinfo() error on hostName=hgdownload.cse.ucsc.edu: No address associated with hostname
No such file or directory
mustOpenFd: Can't open phastCons46way.wib to read
On Wed 02 Jul 2014 03:40:57 PM EDT, Brian Lee wrote:
Dear Kipper,
Thank you for using the UCSC Genome Browser and your helpful feedback,
we have updated the wikipage to include recommendations to add the
gbdbLoc1= path lines.
If you have any further questions or suggestions, please reply to
gen...@soe.ucsc.edu <mailto:gen...@soe.ucsc.edu>. All messages sent to
that address are archived on a publicly-accessible forum. If your
question includes sensitive data, you may send it instead to
genom...@soe.ucsc.edu <mailto:genom...@soe.ucsc.edu>.
All the best,
Brian Lee
On Wed, Jul 2, 2014 at 6:26 AM, Kipper Fletez-Brant<caflet...@gmail.com <mailto:cafletezbrant@gmail.com>> wrote:
I'm writing this so that anyone else having the same problem that
I had can get up to speed.
If you find yourself having gone through all of the steps in the
set of quoted emails and things still don't work, add the
following 2 lines to your .hg.conf:
gbdbLoc1=/path/to/relevant/__WIB/file/
gbdbLoc2=http://hgdownload.__cse.ucsc.edu/gbdb/
<http://hgdownload.cse.ucsc.edu/gbdb/>
This seemed to fix it for me (I discovered this by grepping the
kent src tree files for mention of the /gbdb/ directory and
discovered the file ex.hg.conf, which explained in great detail
many other possible settings)
On 07/01/2014 09:26 AM, Kipper Fletez-Brant wrote:
Hi Luvina,
I must be missing something simple. I have downloaded the
necessary .wib file (phastCons46way.wib in my case; link
provided below), and just to be sure I double checked that my
.hg.conf matches that of your website (I'm listing it below
for completeness), and when I go to run something simple I get
the error listed below.
Please advise,
Kipper
# wib I downloaded
db.host=genome-mysql.cse.ucsc.__edu
<http://genome-mysql.cse.ucsc.edu>
db.user=genomep
db.password=password
central.db=hgcentral
# command and error, called from directory containing
phastCons46way.wib - why doesn't it use the local copy?
hgWiggle -db=hg19 -chr=chr22 phastCons46way
No such file or directory
mustOpenFd: Can't open
/gbdb/hg19/multiz46way/__phastCons46way.wib to read
On Mon 30 Jun 2014 04:30:35 PM EDT, Luvina Guruvadoo wrote:
Hello Kipper,
Thank you for your question. In order to run hgWiggle, you
will need
to download the .wib file into a local directory. Please
refer to our
instructions here:
<mailto:gen...@soe.ucsc.edu <mailto:gen...@soe.ucsc.edu>>.
All messages sent to that address are
archived on a publicly-accessible forum. If your question
includes
sensitive data, you may send it instead to
genom...@soe.ucsc.edu <mailto:genom...@soe.ucsc.edu>
<mailto:genom...@soe.ucsc.__edu
<mailto:genom...@soe.ucsc.edu>>.
- - -
Luvina Guruvadoo
UCSC Genome Bioinformatics Group
On Sat, Jun 28, 2014 at 2:43 PM, Kipper Fletez-Brant
<caflet...@gmail.com <mailto:cafletezbrant@gmail.com>
<mailto:cafletezbrant@gmail.__com
<mailto:cafletezbrant@gmail.com>>> wrote:
Hi,
I am trying to use hgWiggle to access the UCSC MySQL
database to
get phastCons scores for regions of the human genome.
Below is
what I have tried and the error message I get.
Basically, even
though I log into the MySQL client and can see the table
phastCons46way, hgWiggle cannot access it. What am I
doing wrong?
Thank you,
Kipper Fletez-Brant
*** Sample code and error messages ***
# attempt 1: just print all info for all bases
userApps/bin/hgWiggle -doStats
-position=chr1:28125-28872 <tel:28125-28872>
<tel:28125-28872 <tel:28125-28872>> -db=hg19
phastCons46way -doAscii
No such file or directory
mustOpenFd: Can't open
/gbdb/hg19/multiz46way/____phastCons46way.wib
to read
# attempt 2: use a sample text file
userApps/bin/hgWiggle -doStats
-bedFile=hgwiggleTest.txt -db=hg19
phastCons46way -doAscii
No such file or directory
mustOpenFd: Can't open
/gbdb/hg19/multiz46way/____phastCons46way.wib
to read
# attempt 3: just write whatever to screen
userApps/bin/hgWiggle -db=hg19
-position=chr1:28125-28872 <tel:28125-28872>
<tel:28125-28872 <tel:28125-28872>> -rawDataOut
-doStats phastCons46way
No such file or directory
mustOpenFd: Can't open
After following your advice, it worked for maybe 300 queries before I started getting the same at if error messages. As I haven't moved the WIB file, I am unclear why I am getting these 'file not found' errors.
I am attempting this now and will report back later. However, I do not expect this to be the solution, as I successfully queried some 15k genomic regions before I started having problems (which was ~3:00 EDT)
On Wed 02 Jul 2014 08:10:27 PM EDT, Brian Lee wrote:
Dear Kipper,
Thank you for your message. You may need to place your wib file in a
directory to match where hgWiggle will look for it, such as
hg19/multiz46way from the working directory you define with your
gbdbLoc1 line.
I added some example steps in the new "Using hgWiggle options"
section:
http://genomewiki.ucsc.edu/index.php/Using_hgWiggle_without_a_database#Using_hgWiggle_options
If you add gbdbLoc1=/home/usrName/work/ to your .hg.conf file, in that
/home/usrName/work/ directory you will want to make a
hg19/multiz46way/ directory, and move your .wib file there.
If you have any further questions or suggestions, please reply to
gen...@soe.ucsc.edu <mailto:gen...@soe.ucsc.edu>. All messages sent to
that address are archived on a publicly-accessible forum. If your
question includes sensitive data, you may send it instead to
genom...@soe.ucsc.edu <mailto:genom...@soe.ucsc.edu>.
All the best,
Brian Lee
On Wed, Jul 2, 2014 at 3:32 PM, Kipper Fletez-Brant
<caflet...@gmail.com <mailto:cafletezbrant@gmail.com>> wrote:
Well, now I am especially confused. As of ~ 3:00 EDT, I started
getting the error message below. I have changed no settings in
any of my programs or config files. Is something happening with
the browser?
mustOpenFd: Can't open phastCons46way.wib to read
getaddrinfo() error on hostName=hgdownload.cse.ucsc.__edu
<http://hgdownload.cse.ucsc.edu>: No address associated with hostname
No such file or directory
mustOpenFd: Can't open phastCons46way.wib to read
On Wed 02 Jul 2014 03:40:57 PM EDT, Brian Lee wrote:
Dear Kipper,
Thank you for using the UCSC Genome Browser and your helpful
feedback,
we have updated the wikipage to include recommendations to add the
gbdbLoc1= path lines.
If you have any further questions or suggestions, please reply to
gen...@soe.ucsc.edu <mailto:gen...@soe.ucsc.edu>
<mailto:gen...@soe.ucsc.edu <mailto:gen...@soe.ucsc.edu>>. All
messages sent to
that address are archived on a publicly-accessible forum. If your
question includes sensitive data, you may send it instead to
genom...@soe.ucsc.edu <mailto:genom...@soe.ucsc.edu>
<mailto:genom...@soe.ucsc.__edu
<mailto:genom...@soe.ucsc.edu>>.
All the best,
Brian Lee
On Wed, Jul 2, 2014 at 6:26 AM, Kipper Fletez-Brant
<caflet...@gmail.com <mailto:cafletezbrant@gmail.com>
<mailto:cafletezbrant@gmail.__com
<mailto:cafletezbrant@gmail.com>>> wrote:
I'm writing this so that anyone else having the same
problem that
I had can get up to speed.
If you find yourself having gone through all of the steps
in the
set of quoted emails and things still don't work, add the
following 2 lines to your .hg.conf:
gbdbLoc1=/path/to/relevant/____WIB/file/
gbdbLoc2=http://hgdownload.__c__se.ucsc.edu/gbdb/
<http://cse.ucsc.edu/gbdb/>
<http://hgdownload.cse.ucsc.__edu/gbdb/
<http://hgdownload.cse.ucsc.edu/gbdb/>>
This seemed to fix it for me (I discovered this by
grepping the
kent src tree files for mention of the /gbdb/ directory and
discovered the file ex.hg.conf, which explained in great
detail
many other possible settings)
On 07/01/2014 09:26 AM, Kipper Fletez-Brant wrote:
Hi Luvina,
I must be missing something simple. I have downloaded the
necessary .wib file (phastCons46way.wib in my case; link
provided below), and just to be sure I double checked
that my
.hg.conf matches that of your website (I'm listing it
below
for completeness), and when I go to run something
simple I get
the error listed below.
Please advise,
Kipper
# wib I downloaded
ftp://hgdownload.cse.ucsc.edu/____gbdb/hg19/multiz46way/____phastCons46way.wib
<ftp://hgdownload.cse.ucsc.edu/__gbdb/hg19/multiz46way/__phastCons46way.wib>
<ftp://hgdownload.cse.ucsc.__edu/gbdb/hg19/multiz46way/__phastCons46way.wib
db.host=genome-mysql.cse.ucsc.____edu
<http://genome-mysql.cse.ucsc.__edu
<http://genome-mysql.cse.ucsc.edu>>
db.user=genomep
db.password=password
central.db=hgcentral
# command and error, called from directory containing
phastCons46way.wib - why doesn't it use the local copy?
hgWiggle -db=hg19 -chr=chr22 phastCons46way
No such file or directory
mustOpenFd: Can't open
/gbdb/hg19/multiz46way/____phastCons46way.wib to read
On Mon 30 Jun 2014 04:30:35 PM EDT, Luvina Guruvadoo
wrote:
Hello Kipper,
Thank you for your question. In order to run
hgWiggle, you
will need
to download the .wib file into a local directory.
Please
refer to our
instructions here:
http://genomewiki.ucsc.edu/____index.php/Using_hgWiggle_____without_a_database
<http://genomewiki.ucsc.edu/__index.php/Using_hgWiggle___without_a_database>
<http://genomewiki.ucsc.edu/__index.php/Using_hgWiggle___without_a_database
<http://genomewiki.ucsc.edu/index.php/Using_hgWiggle_without_a_database>>.
If you have any further questions, please reply to
gen...@soe.ucsc.edu <mailto:gen...@soe.ucsc.edu>
<mailto:gen...@soe.ucsc.edu <mailto:gen...@soe.ucsc.edu>>
<mailto:gen...@soe.ucsc.edu
<mailto:gen...@soe.ucsc.edu> <mailto:gen...@soe.ucsc.edu
<mailto:gen...@soe.ucsc.edu>>>.
All messages sent to that address are
archived on a publicly-accessible forum. If your
question
includes
sensitive data, you may send it instead to
genom...@soe.ucsc.edu <mailto:genom...@soe.ucsc.edu>
<mailto:genom...@soe.ucsc.__edu
<mailto:genom...@soe.ucsc.edu>>
<mailto:genom...@soe.ucsc.
<mailto:genom...@soe.ucsc.>____edu
<mailto:genom...@soe.ucsc.__edu
<mailto:genom...@soe.ucsc.edu>>>.
- - -
Luvina Guruvadoo
UCSC Genome Bioinformatics Group
On Sat, Jun 28, 2014 at 2:43 PM, Kipper Fletez-Brant
<caflet...@gmail.com
<mailto:cafletezbrant@gmail.com>
<mailto:cafletezbrant@gmail.__com
<mailto:cafletezbrant@gmail.com>>
<mailto:cafletezbrant@gmail.
<mailto:cafletezbrant@gmail.>____com
/gbdb/hg19/multiz46way/______phastCons46way.wib
to read
# attempt 2: use a sample text file
userApps/bin/hgWiggle -doStats
-bedFile=hgwiggleTest.txt -db=hg19
phastCons46way -doAscii
No such file or directory
mustOpenFd: Can't open
/gbdb/hg19/multiz46way/______phastCons46way.wib
to read
# attempt 3: just write whatever to screen
userApps/bin/hgWiggle -db=hg19
-position=chr1:28125-28872 <tel:28125-28872>
<tel:28125-28872 <tel:28125-28872>>
<tel:28125-28872 <tel:28125-28872>
<tel:28125-28872 <tel:28125-28872>>> -rawDataOut
-doStats phastCons46way
No such file or directory
mustOpenFd: Can't open
/gbdb/hg19/multiz46way/______phastCons46way.wib
to read
--
--
<caflet...@gmail.com <mailto:caflet...@gmail.com>
<mailto:cafletezbrant@gmail.__com
<mailto:caflet...@gmail.com>
<mailto:cafletezbrant@gmail.__com
<mailto:caflet...@gmail.com>>
<mailto:cafletezbrant@gmail.
<mailto:cafletezbrant@gmail.>____com
<mailto:cafletezbrant@gmail.__com
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