hgWiggle Cannot Find phastCons46way

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Kipper Fletez-Brant

unread,
Jun 30, 2014, 11:30:28 AM6/30/14
to gen...@soe.ucsc.edu
Hi,

I am trying to use hgWiggle to access the UCSC MySQL database to get
phastCons scores for regions of the human genome. Below is what I have
tried and the error message I get. Basically, even though I log into
the MySQL client and can see the table phastCons46way, hgWiggle cannot
access it. What am I doing wrong?

Thank you,
Kipper Fletez-Brant

*** Sample code and error messages ***

# attempt 1: just print all info for all bases
userApps/bin/hgWiggle -doStats -position=chr1:28125-28872 -db=hg19
phastCons46way -doAscii
No such file or directory
mustOpenFd: Can't open /gbdb/hg19/multiz46way/phastCons46way.wib to read

# attempt 2: use a sample text file
userApps/bin/hgWiggle -doStats -bedFile=hgwiggleTest.txt -db=hg19
phastCons46way -doAscii
No such file or directory
mustOpenFd: Can't open /gbdb/hg19/multiz46way/phastCons46way.wib to read

# attempt 3: just write whatever to screen
userApps/bin/hgWiggle -db=hg19 -position=chr1:28125-28872 -rawDataOut
-doStats phastCons46way
No such file or directory
mustOpenFd: Can't open /gbdb/hg19/multiz46way/phastCons46way.wib to read

Luvina Guruvadoo

unread,
Jun 30, 2014, 4:30:38 PM6/30/14
to Kipper Fletez-Brant, gen...@soe.ucsc.edu
Hello Kipper,

Thank you for your question. In order to run hgWiggle, you will need to download the .wib file into a local directory. Please refer to our instructions here: http://genomewiki.ucsc.edu/index.php/Using_hgWiggle_without_a_database.

If you have any further questions, please reply to gen...@soe.ucsc.edu. All messages sent to that address are archived on a publicly-accessible forum. If your question includes sensitive data, you may send it instead to genom...@soe.ucsc.edu.

- - -
Luvina Guruvadoo
UCSC Genome Bioinformatics Group





--


Kipper Fletez-Brant

unread,
Jul 1, 2014, 11:27:04 AM7/1/14
to Luvina Guruvadoo, gen...@soe.ucsc.edu
Hi Luvina,

I must be missing something simple. I have downloaded the necessary
.wib file (phastCons46way.wib in my case; link provided below), and
just to be sure I double checked that my .hg.conf matches that of your
website (I'm listing it below for completeness), and when I go to run
something simple I get the error listed below.

Please advise,
Kipper

# wib I downloaded
ftp://hgdownload.cse.ucsc.edu/gbdb/hg19/multiz46way/phastCons46way.wib

# my .hg.conf
db.host=genome-mysql.cse.ucsc.edu
db.user=genomep
db.password=password
central.db=hgcentral

# command and error, called from directory containing
phastCons46way.wib - why doesn't it use the local copy?
hgWiggle -db=hg19 -chr=chr22 phastCons46way
No such file or directory
mustOpenFd: Can't open /gbdb/hg19/multiz46way/phastCons46way.wib to read




On Mon 30 Jun 2014 04:30:35 PM EDT, Luvina Guruvadoo wrote:
> Hello Kipper,
>
> Thank you for your question. In order to run hgWiggle, you will need
> to download the .wib file into a local directory. Please refer to our
> instructions here:
> http://genomewiki.ucsc.edu/index.php/Using_hgWiggle_without_a_database.
>
> If you have any further questions, please reply to gen...@soe.ucsc.edu
> <mailto:gen...@soe.ucsc.edu>. All messages sent to that address are
> archived on a publicly-accessible forum. If your question includes
> sensitive data, you may send it instead to genom...@soe.ucsc.edu
> <mailto:genom...@soe.ucsc.edu>.
>
> - - -
> Luvina Guruvadoo
> UCSC Genome Bioinformatics Group
>
>
>
> On Sat, Jun 28, 2014 at 2:43 PM, Kipper Fletez-Brant
> <caflet...@gmail.com <mailto:caflet...@gmail.com>> wrote:
>
> Hi,
>
> I am trying to use hgWiggle to access the UCSC MySQL database to
> get phastCons scores for regions of the human genome. Below is
> what I have tried and the error message I get. Basically, even
> though I log into the MySQL client and can see the table
> phastCons46way, hgWiggle cannot access it. What am I doing wrong?
>
> Thank you,
> Kipper Fletez-Brant
>
> *** Sample code and error messages ***
>
> # attempt 1: just print all info for all bases
> userApps/bin/hgWiggle -doStats -position=chr1:28125-28872
> <tel:28125-28872> -db=hg19 phastCons46way -doAscii
> No such file or directory
> mustOpenFd: Can't open /gbdb/hg19/multiz46way/__phastCons46way.wib
> to read
>
> # attempt 2: use a sample text file
> userApps/bin/hgWiggle -doStats -bedFile=hgwiggleTest.txt -db=hg19
> phastCons46way -doAscii
> No such file or directory
> mustOpenFd: Can't open /gbdb/hg19/multiz46way/__phastCons46way.wib
> to read
>
> # attempt 3: just write whatever to screen
> userApps/bin/hgWiggle -db=hg19 -position=chr1:28125-28872
> <tel:28125-28872> -rawDataOut -doStats phastCons46way
> No such file or directory
> mustOpenFd: Can't open /gbdb/hg19/multiz46way/__phastCons46way.wib
> to read
>
> --
>
>

Kipper Fletez-Brant

unread,
Jul 2, 2014, 11:28:24 AM7/2/14
to Luvina Guruvadoo, gen...@soe.ucsc.edu
I'm writing this so that anyone else having the same problem that I had
can get up to speed.

If you find yourself having gone through all of the steps in the set of
quoted emails and things still don't work, add the following 2 lines to
your .hg.conf:

gbdbLoc1=/path/to/relevant/WIB/file/
gbdbLoc2=http://hgdownload.cse.ucsc.edu/gbdb/

This seemed to fix it for me (I discovered this by grepping the kent src
tree files for mention of the /gbdb/ directory and discovered the file
ex.hg.conf, which explained in great detail many other possible settings)

Brian Lee

unread,
Jul 2, 2014, 3:41:55 PM7/2/14
to Kipper Fletez-Brant, Luvina Guruvadoo, gen...@soe.ucsc.edu
Dear Kipper,

Thank you for using the UCSC Genome Browser and your helpful feedback, we have updated the wikipage to include recommendations to add the gbdbLoc1= path lines.

If you have any further questions or suggestions, please reply to gen...@soe.ucsc.edu. All messages sent to that address are archived on a publicly-accessible forum. If your question includes sensitive data, you may send it instead to genom...@soe.ucsc.edu.

All the best,
Brian Lee



--


Kipper Fletez-Brant

unread,
Jul 2, 2014, 6:46:15 PM7/2/14
to Brian Lee, Luvina Guruvadoo, gen...@soe.ucsc.edu
Sorry, hit 'Send' too soon. The error message continues:

Couldn't connect to database hg19 on genome-mysql.cse.ucsc.edu as
genomep.
Unknown MySQL server host 'genome-mysql.cse.ucsc.edu' (2)

Can't get data socket for
http://hgdownload.cse.ucsc.edu/gbdb/hg19/multiz46way/phastCons46way.wib
getaddrinfo() error on hostName=hgdownload.cse.ucsc.edu: No address
associated with hostname



On Wed 02 Jul 2014 06:32:20 PM EDT, Kipper Fletez-Brant wrote:
> Well, now I am especially confused. As of ~ 3:00 EDT, I started
> getting the error message below. I have changed no settings in any of
> my programs or config files. Is something happening with the browser?
>
> mustOpenFd: Can't open phastCons46way.wib to read
> getaddrinfo() error on hostName=hgdownload.cse.ucsc.edu: No address
> associated with hostname
>
> No such file or directory
> mustOpenFd: Can't open phastCons46way.wib to read
>
>
>
> On Wed 02 Jul 2014 03:40:57 PM EDT, Brian Lee wrote:
>> Dear Kipper,
>>
>> Thank you for using the UCSC Genome Browser and your helpful feedback,
>> we have updated the wikipage to include recommendations to add the
>> gbdbLoc1= path lines.
>>
>> If you have any further questions or suggestions, please reply to
>> gen...@soe.ucsc.edu <mailto:gen...@soe.ucsc.edu>. All messages sent to
>> that address are archived on a publicly-accessible forum. If your
>> question includes sensitive data, you may send it instead to
>> genom...@soe.ucsc.edu <mailto:genom...@soe.ucsc.edu>.
>>
>> All the best,
>> Brian Lee
>>
>>
>>
>> On Wed, Jul 2, 2014 at 6:26 AM, Kipper Fletez-Brant
>> <caflet...@gmail.com <mailto:caflet...@gmail.com>> wrote:
>>
>> I'm writing this so that anyone else having the same problem that
>> I had can get up to speed.
>>
>> If you find yourself having gone through all of the steps in the
>> set of quoted emails and things still don't work, add the
>> following 2 lines to your .hg.conf:
>>
>> gbdbLoc1=/path/to/relevant/__WIB/file/
>> gbdbLoc2=http://hgdownload.__cse.ucsc.edu/gbdb/
>> <http://hgdownload.cse.ucsc.edu/gbdb/>
>>
>> This seemed to fix it for me (I discovered this by grepping the
>> kent src tree files for mention of the /gbdb/ directory and
>> discovered the file ex.hg.conf, which explained in great detail
>> many other possible settings)
>>
>>
>> On 07/01/2014 09:26 AM, Kipper Fletez-Brant wrote:
>>
>> Hi Luvina,
>>
>> I must be missing something simple. I have downloaded the
>> necessary .wib file (phastCons46way.wib in my case; link
>> provided below), and just to be sure I double checked that my
>> .hg.conf matches that of your website (I'm listing it below
>> for completeness), and when I go to run something simple I get
>> the error listed below.
>>
>> Please advise,
>> Kipper
>>
>> # wib I downloaded
>>
>> ftp://hgdownload.cse.ucsc.edu/__gbdb/hg19/multiz46way/__phastCons46way.wib
>> db.host=genome-mysql.cse.ucsc.__edu
>> <http://genome-mysql.cse.ucsc.edu>
>> db.user=genomep
>> db.password=password
>> central.db=hgcentral
>>
>> # command and error, called from directory containing
>> phastCons46way.wib - why doesn't it use the local copy?
>> hgWiggle -db=hg19 -chr=chr22 phastCons46way
>> No such file or directory
>> mustOpenFd: Can't open
>> /gbdb/hg19/multiz46way/__phastCons46way.wib to read
>>
>>
>>
>>
>> On Mon 30 Jun 2014 04:30:35 PM EDT, Luvina Guruvadoo wrote:
>>
>> Hello Kipper,
>>
>> Thank you for your question. In order to run hgWiggle, you
>> will need
>> to download the .wib file into a local directory. Please
>> refer to our
>> instructions here:
>>
>> http://genomewiki.ucsc.edu/__index.php/Using_hgWiggle___without_a_database
>>
>>
>> <http://genomewiki.ucsc.edu/index.php/Using_hgWiggle_without_a_database>.
>>
>>
>> If you have any further questions, please reply to
>> gen...@soe.ucsc.edu <mailto:gen...@soe.ucsc.edu>
>> <mailto:gen...@soe.ucsc.edu <mailto:gen...@soe.ucsc.edu>>.
>> All messages sent to that address are
>> archived on a publicly-accessible forum. If your question
>> includes
>> sensitive data, you may send it instead to
>> genom...@soe.ucsc.edu <mailto:genom...@soe.ucsc.edu>
>> <mailto:genom...@soe.ucsc.__edu
>> <mailto:genom...@soe.ucsc.edu>>.
>>
>> - - -
>> Luvina Guruvadoo
>> UCSC Genome Bioinformatics Group
>>
>>
>>
>> On Sat, Jun 28, 2014 at 2:43 PM, Kipper Fletez-Brant
>> <caflet...@gmail.com <mailto:caflet...@gmail.com>
>> <mailto:cafletezbrant@gmail.__com
>> <mailto:caflet...@gmail.com>>> wrote:
>>
>> Hi,
>>
>> I am trying to use hgWiggle to access the UCSC MySQL
>> database to
>> get phastCons scores for regions of the human genome.
>> Below is
>> what I have tried and the error message I get.
>> Basically, even
>> though I log into the MySQL client and can see the table
>> phastCons46way, hgWiggle cannot access it. What am I
>> doing wrong?
>>
>> Thank you,
>> Kipper Fletez-Brant
>>
>> *** Sample code and error messages ***
>>
>> # attempt 1: just print all info for all bases
>> userApps/bin/hgWiggle -doStats
>> -position=chr1:28125-28872 <tel:28125-28872>
>> <tel:28125-28872 <tel:28125-28872>> -db=hg19
>> phastCons46way -doAscii
>> No such file or directory
>> mustOpenFd: Can't open
>> /gbdb/hg19/multiz46way/____phastCons46way.wib
>> to read
>>
>> # attempt 2: use a sample text file
>> userApps/bin/hgWiggle -doStats
>> -bedFile=hgwiggleTest.txt -db=hg19
>> phastCons46way -doAscii
>> No such file or directory
>> mustOpenFd: Can't open
>> /gbdb/hg19/multiz46way/____phastCons46way.wib
>> to read
>>
>> # attempt 3: just write whatever to screen
>> userApps/bin/hgWiggle -db=hg19
>> -position=chr1:28125-28872 <tel:28125-28872>
>> <tel:28125-28872 <tel:28125-28872>> -rawDataOut
>> -doStats phastCons46way
>> No such file or directory
>> mustOpenFd: Can't open
>> /gbdb/hg19/multiz46way/____phastCons46way.wib
>> to read
>>
>> --
>>
>>
>>
>> --
>>
>>

Brian Lee

unread,
Jul 2, 2014, 8:10:49 PM7/2/14
to Kipper Fletez-Brant, Luvina Guruvadoo, gen...@soe.ucsc.edu
Dear Kipper,

Thank you for your message.  You may need to place your wib file in a directory to match where hgWiggle will look for it, such as hg19/multiz46way from the working directory you define with your gbdbLoc1 line.

I added some example steps in the new "Using hgWiggle options" section: http://genomewiki.ucsc.edu/index.php/Using_hgWiggle_without_a_database#Using_hgWiggle_options

If you add gbdbLoc1=/home/usrName/work/ to your .hg.conf file, in that /home/usrName/work/ directory you will want to make a hg19/multiz46way/ directory, and move your .wib file there.

If you have any further questions or suggestions, please reply to gen...@soe.ucsc.edu. All messages sent to that address are archived on a publicly-accessible forum. If your question includes sensitive data, you may send it instead to genom...@soe.ucsc.edu.
All the best,
Brian Lee

On Wed, Jul 2, 2014 at 3:32 PM, Kipper Fletez-Brant <caflet...@gmail.com> wrote:
Well, now I am especially confused.  As of ~ 3:00 EDT, I started getting the error message below.  I have changed no settings in any of my programs or config files.  Is something happening with the browser?

mustOpenFd: Can't open phastCons46way.wib to read
getaddrinfo() error on hostName=hgdownload.cse.ucsc.edu: No address associated with hostname
No such file or directory
mustOpenFd: Can't open phastCons46way.wib to read




On Wed 02 Jul 2014 03:40:57 PM EDT, Brian Lee wrote:
Dear Kipper,

Thank you for using the UCSC Genome Browser and your helpful feedback,
we have updated the wikipage to include recommendations to add the
gbdbLoc1= path lines.

If you have any further questions or suggestions, please reply to
gen...@soe.ucsc.edu <mailto:gen...@soe.ucsc.edu>. All messages sent to
that address are archived on a publicly-accessible forum. If your
question includes sensitive data, you may send it instead to
genom...@soe.ucsc.edu <mailto:genom...@soe.ucsc.edu>.

All the best,
Brian Lee



On Wed, Jul 2, 2014 at 6:26 AM, Kipper Fletez-Brant
<caflet...@gmail.com <mailto:cafletezbrant@gmail.com>> wrote:

    I'm writing this so that anyone else having the same problem that
    I had can get up to speed.

    If you find yourself having gone through all of the steps in the
    set of quoted emails and things still don't work, add the
    following 2 lines to your .hg.conf:

     gbdbLoc1=/path/to/relevant/__WIB/file/
     gbdbLoc2=http://hgdownload.__cse.ucsc.edu/gbdb/

    <http://hgdownload.cse.ucsc.edu/gbdb/>

    This seemed to fix it for me (I discovered this by grepping the
    kent src tree files for mention of the /gbdb/ directory and
    discovered the file ex.hg.conf, which explained in great detail
    many other possible settings)


    On 07/01/2014 09:26 AM, Kipper Fletez-Brant wrote:

        Hi Luvina,

        I must be missing something simple.  I have downloaded the
        necessary .wib file (phastCons46way.wib in my case; link
        provided below), and just to be sure I double checked that my
        .hg.conf matches that of your website (I'm listing it below
        for completeness), and when I go to run something simple I get
        the error listed below.

        Please advise,
        Kipper

        # wib I downloaded
        db.host=genome-mysql.cse.ucsc.__edu
        <http://genome-mysql.cse.ucsc.edu>

        db.user=genomep
        db.password=password
        central.db=hgcentral

        # command and error, called from directory containing
        phastCons46way.wib - why doesn't it use the local copy?
        hgWiggle -db=hg19 -chr=chr22 phastCons46way
        No such file or directory
        mustOpenFd: Can't open
        /gbdb/hg19/multiz46way/__phastCons46way.wib to read





        On Mon 30 Jun 2014 04:30:35 PM EDT, Luvina Guruvadoo wrote:

            Hello Kipper,

            Thank you for your question. In order to run hgWiggle, you
            will need
            to download the .wib file into a local directory. Please
            refer to our
            instructions here:


            If you have any further questions, please reply to
            gen...@soe.ucsc.edu <mailto:gen...@soe.ucsc.edu>
            <mailto:gen...@soe.ucsc.edu <mailto:gen...@soe.ucsc.edu>>.

            All messages sent to that address are
            archived on a publicly-accessible forum. If your question
            includes
            sensitive data, you may send it instead to
            genom...@soe.ucsc.edu <mailto:genom...@soe.ucsc.edu>
            <mailto:genom...@soe.ucsc.__edu

            <mailto:genom...@soe.ucsc.edu>>.

            - - -
            Luvina Guruvadoo
            UCSC Genome Bioinformatics Group



            On Sat, Jun 28, 2014 at 2:43 PM, Kipper Fletez-Brant
            <caflet...@gmail.com <mailto:cafletezbrant@gmail.com>
            <mailto:cafletezbrant@gmail.__com

            <mailto:cafletezbrant@gmail.com>>> wrote:

                Hi,

                I am trying to use hgWiggle to access the UCSC MySQL
            database to
                get phastCons scores for regions of the human genome.
             Below is
                what I have tried and the error message I get.
             Basically, even
                though I log into the MySQL client and can see the table
                phastCons46way, hgWiggle cannot access it.  What am I
            doing wrong?

                Thank you,
                Kipper Fletez-Brant

                *** Sample code and error messages ***

                # attempt 1: just print all info for all bases
                userApps/bin/hgWiggle -doStats
            -position=chr1:28125-28872 <tel:28125-28872>
                <tel:28125-28872 <tel:28125-28872>> -db=hg19

            phastCons46way -doAscii
                No such file or directory
                mustOpenFd: Can't open
            /gbdb/hg19/multiz46way/____phastCons46way.wib

                to read

                # attempt 2: use a sample text file
                userApps/bin/hgWiggle -doStats
            -bedFile=hgwiggleTest.txt -db=hg19
                phastCons46way -doAscii
                No such file or directory
                mustOpenFd: Can't open
            /gbdb/hg19/multiz46way/____phastCons46way.wib

                to read

                # attempt 3: just write whatever to screen
                userApps/bin/hgWiggle -db=hg19
            -position=chr1:28125-28872 <tel:28125-28872>
                <tel:28125-28872 <tel:28125-28872>> -rawDataOut

            -doStats phastCons46way
                No such file or directory
                mustOpenFd: Can't open

Kipper Fletez-Brant

unread,
Jul 3, 2014, 11:43:07 AM7/3/14
to Brian Lee, gen...@soe.ucsc.edu, Luvina Guruvadoo

After following your advice, it worked for maybe 300 queries before I started getting the same at if error messages. As I haven't moved the WIB file, I am unclear why I am getting these 'file not found' errors.

On Jul 2, 2014 9:16 PM, "Kipper Fletez-Brant" <caflet...@gmail.com> wrote:
I am attempting this now and will report back later.  However, I do not expect this to be the solution, as I successfully queried some 15k genomic regions before I started having problems (which was ~3:00 EDT)


On Wed 02 Jul 2014 08:10:27 PM EDT, Brian Lee wrote:
Dear Kipper,

Thank you for your message.  You may need to place your wib file in a
directory to match where hgWiggle will look for it, such as
hg19/multiz46way from the working directory you define with your
gbdbLoc1 line.

I added some example steps in the new "Using hgWiggle options"
section:
http://genomewiki.ucsc.edu/index.php/Using_hgWiggle_without_a_database#Using_hgWiggle_options

If you add gbdbLoc1=/home/usrName/work/ to your .hg.conf file, in that
/home/usrName/work/ directory you will want to make a
hg19/multiz46way/ directory, and move your .wib file there.

If you have any further questions or suggestions, please reply to
gen...@soe.ucsc.edu <mailto:gen...@soe.ucsc.edu>. All messages sent to
that address are archived on a publicly-accessible forum. If your
question includes sensitive data, you may send it instead to
genom...@soe.ucsc.edu <mailto:genom...@soe.ucsc.edu>.
All the best,
Brian Lee



On Wed, Jul 2, 2014 at 3:32 PM, Kipper Fletez-Brant
<caflet...@gmail.com <mailto:cafletezbrant@gmail.com>> wrote:

    Well, now I am especially confused.  As of ~ 3:00 EDT, I started
    getting the error message below.  I have changed no settings in
    any of my programs or config files.  Is something happening with
    the browser?

    mustOpenFd: Can't open phastCons46way.wib to read
    getaddrinfo() error on hostName=hgdownload.cse.ucsc.__edu
    <http://hgdownload.cse.ucsc.edu>: No address associated with hostname



    No such file or directory
    mustOpenFd: Can't open phastCons46way.wib to read




    On Wed 02 Jul 2014 03:40:57 PM EDT, Brian Lee wrote:

        Dear Kipper,

        Thank you for using the UCSC Genome Browser and your helpful
        feedback,
        we have updated the wikipage to include recommendations to add the
        gbdbLoc1= path lines.

        If you have any further questions or suggestions, please reply to
        gen...@soe.ucsc.edu <mailto:gen...@soe.ucsc.edu>
        <mailto:gen...@soe.ucsc.edu <mailto:gen...@soe.ucsc.edu>>. All
        messages sent to
        that address are archived on a publicly-accessible forum. If your
        question includes sensitive data, you may send it instead to
        genom...@soe.ucsc.edu <mailto:genom...@soe.ucsc.edu>
        <mailto:genom...@soe.ucsc.__edu
        <mailto:genom...@soe.ucsc.edu>>.

        All the best,
        Brian Lee



        On Wed, Jul 2, 2014 at 6:26 AM, Kipper Fletez-Brant
        <caflet...@gmail.com <mailto:cafletezbrant@gmail.com>
        <mailto:cafletezbrant@gmail.__com
        <mailto:cafletezbrant@gmail.com>>> wrote:

            I'm writing this so that anyone else having the same
        problem that
            I had can get up to speed.

            If you find yourself having gone through all of the steps
        in the
            set of quoted emails and things still don't work, add the
            following 2 lines to your .hg.conf:

             gbdbLoc1=/path/to/relevant/____WIB/file/
             gbdbLoc2=http://hgdownload.__c__se.ucsc.edu/gbdb/
        <http://cse.ucsc.edu/gbdb/>

            <http://hgdownload.cse.ucsc.__edu/gbdb/

        <http://hgdownload.cse.ucsc.edu/gbdb/>>

            This seemed to fix it for me (I discovered this by
        grepping the
            kent src tree files for mention of the /gbdb/ directory and
            discovered the file ex.hg.conf, which explained in great
        detail
            many other possible settings)


            On 07/01/2014 09:26 AM, Kipper Fletez-Brant wrote:

                Hi Luvina,

                I must be missing something simple.  I have downloaded the
                necessary .wib file (phastCons46way.wib in my case; link
                provided below), and just to be sure I double checked
        that my
                .hg.conf matches that of your website (I'm listing it
        below
                for completeness), and when I go to run something
        simple I get
                the error listed below.

                Please advise,
                Kipper

                # wib I downloaded

        <ftp://hgdownload.cse.ucsc.edu/gbdb/hg19/multiz46way/phastCons46way.wib>>

                # my .hg.conf
                db.host=genome-mysql.cse.ucsc.____edu
                <http://genome-mysql.cse.ucsc.__edu

        <http://genome-mysql.cse.ucsc.edu>>

                db.user=genomep
                db.password=password
                central.db=hgcentral

                # command and error, called from directory containing
                phastCons46way.wib - why doesn't it use the local copy?
                hgWiggle -db=hg19 -chr=chr22 phastCons46way
                No such file or directory
                mustOpenFd: Can't open
                /gbdb/hg19/multiz46way/____phastCons46way.wib to read






                On Mon 30 Jun 2014 04:30:35 PM EDT, Luvina Guruvadoo
        wrote:

                    Hello Kipper,

                    Thank you for your question. In order to run
        hgWiggle, you
                    will need
                    to download the .wib file into a local directory.
        Please
                    refer to our
                    instructions here:


        <http://genomewiki.ucsc.edu/__index.php/Using_hgWiggle___without_a_database
        <http://genomewiki.ucsc.edu/index.php/Using_hgWiggle_without_a_database>>.


                    If you have any further questions, please reply to
        gen...@soe.ucsc.edu <mailto:gen...@soe.ucsc.edu>
        <mailto:gen...@soe.ucsc.edu <mailto:gen...@soe.ucsc.edu>>
                    <mailto:gen...@soe.ucsc.edu
        <mailto:gen...@soe.ucsc.edu> <mailto:gen...@soe.ucsc.edu
        <mailto:gen...@soe.ucsc.edu>>>.

                    All messages sent to that address are
                    archived on a publicly-accessible forum. If your
        question
                    includes
                    sensitive data, you may send it instead to
        genom...@soe.ucsc.edu <mailto:genom...@soe.ucsc.edu>
        <mailto:genom...@soe.ucsc.__edu
        <mailto:genom...@soe.ucsc.edu>>
                    <mailto:genom...@soe.ucsc.
        <mailto:genom...@soe.ucsc.>____edu


                    <mailto:genom...@soe.ucsc.__edu
        <mailto:genom...@soe.ucsc.edu>>>.

                    - - -
                    Luvina Guruvadoo
                    UCSC Genome Bioinformatics Group



                    On Sat, Jun 28, 2014 at 2:43 PM, Kipper Fletez-Brant
                    <caflet...@gmail.com
        <mailto:cafletezbrant@gmail.com>
        <mailto:cafletezbrant@gmail.__com
        <mailto:cafletezbrant@gmail.com>>
                    <mailto:cafletezbrant@gmail.
        <mailto:cafletezbrant@gmail.>____com
                    /gbdb/hg19/multiz46way/______phastCons46way.wib


                        to read

                        # attempt 2: use a sample text file
                        userApps/bin/hgWiggle -doStats
                    -bedFile=hgwiggleTest.txt -db=hg19
                        phastCons46way -doAscii
                        No such file or directory
                        mustOpenFd: Can't open
                    /gbdb/hg19/multiz46way/______phastCons46way.wib


                        to read

                        # attempt 3: just write whatever to screen
                        userApps/bin/hgWiggle -db=hg19
                    -position=chr1:28125-28872 <tel:28125-28872>
        <tel:28125-28872 <tel:28125-28872>>
                        <tel:28125-28872 <tel:28125-28872>

        <tel:28125-28872 <tel:28125-28872>>> -rawDataOut

                    -doStats phastCons46way
                        No such file or directory
                        mustOpenFd: Can't open
                    /gbdb/hg19/multiz46way/______phastCons46way.wib
                        to read

                        --



            --



Kipper Fletez-Brant

unread,
Jul 3, 2014, 11:43:07 AM7/3/14
to Brian Lee, Luvina Guruvadoo, gen...@soe.ucsc.edu
I am attempting this now and will report back later. However, I do not
expect this to be the solution, as I successfully queried some 15k
genomic regions before I started having problems (which was ~3:00 EDT)

On Wed 02 Jul 2014 08:10:27 PM EDT, Brian Lee wrote:
> Dear Kipper,
>
> Thank you for your message. You may need to place your wib file in a
> directory to match where hgWiggle will look for it, such as
> hg19/multiz46way from the working directory you define with your
> gbdbLoc1 line.
>
> I added some example steps in the new "Using hgWiggle options"
> section:
> http://genomewiki.ucsc.edu/index.php/Using_hgWiggle_without_a_database#Using_hgWiggle_options
>
> If you add gbdbLoc1=/home/usrName/work/ to your .hg.conf file, in that
> /home/usrName/work/ directory you will want to make a
> hg19/multiz46way/ directory, and move your .wib file there.
>
> If you have any further questions or suggestions, please reply to
> gen...@soe.ucsc.edu <mailto:gen...@soe.ucsc.edu>. All messages sent to
> that address are archived on a publicly-accessible forum. If your
> question includes sensitive data, you may send it instead to
> genom...@soe.ucsc.edu <mailto:genom...@soe.ucsc.edu>.
> All the best,
> Brian Lee
>
>
>
> On Wed, Jul 2, 2014 at 3:32 PM, Kipper Fletez-Brant
> <caflet...@gmail.com <mailto:caflet...@gmail.com>> wrote:
>
> Well, now I am especially confused. As of ~ 3:00 EDT, I started
> getting the error message below. I have changed no settings in
> any of my programs or config files. Is something happening with
> the browser?
>
> mustOpenFd: Can't open phastCons46way.wib to read
> getaddrinfo() error on hostName=hgdownload.cse.ucsc.__edu
> <http://hgdownload.cse.ucsc.edu>: No address associated with hostname
>
>
> No such file or directory
> mustOpenFd: Can't open phastCons46way.wib to read
>
>
>
>
> On Wed 02 Jul 2014 03:40:57 PM EDT, Brian Lee wrote:
>
> Dear Kipper,
>
> Thank you for using the UCSC Genome Browser and your helpful
> feedback,
> we have updated the wikipage to include recommendations to add the
> gbdbLoc1= path lines.
>
> If you have any further questions or suggestions, please reply to
> gen...@soe.ucsc.edu <mailto:gen...@soe.ucsc.edu>
> <mailto:gen...@soe.ucsc.edu <mailto:gen...@soe.ucsc.edu>>. All
> messages sent to
> that address are archived on a publicly-accessible forum. If your
> question includes sensitive data, you may send it instead to
> genom...@soe.ucsc.edu <mailto:genom...@soe.ucsc.edu>
> <mailto:genom...@soe.ucsc.__edu
> <mailto:genom...@soe.ucsc.edu>>.
>
> All the best,
> Brian Lee
>
>
>
> On Wed, Jul 2, 2014 at 6:26 AM, Kipper Fletez-Brant
> <mailto:caflet...@gmail.com>>> wrote:
>
> I'm writing this so that anyone else having the same
> problem that
> I had can get up to speed.
>
> If you find yourself having gone through all of the steps
> in the
> set of quoted emails and things still don't work, add the
> following 2 lines to your .hg.conf:
>
> gbdbLoc1=/path/to/relevant/____WIB/file/
> gbdbLoc2=http://hgdownload.__c__se.ucsc.edu/gbdb/
> <http://cse.ucsc.edu/gbdb/>
>
> <http://hgdownload.cse.ucsc.__edu/gbdb/
> <http://hgdownload.cse.ucsc.edu/gbdb/>>
>
> This seemed to fix it for me (I discovered this by
> grepping the
> kent src tree files for mention of the /gbdb/ directory and
> discovered the file ex.hg.conf, which explained in great
> detail
> many other possible settings)
>
>
> On 07/01/2014 09:26 AM, Kipper Fletez-Brant wrote:
>
> Hi Luvina,
>
> I must be missing something simple. I have downloaded the
> necessary .wib file (phastCons46way.wib in my case; link
> provided below), and just to be sure I double checked
> that my
> .hg.conf matches that of your website (I'm listing it
> below
> for completeness), and when I go to run something
> simple I get
> the error listed below.
>
> Please advise,
> Kipper
>
> # wib I downloaded
> db.host=genome-mysql.cse.ucsc.____edu
> <http://genome-mysql.cse.ucsc.__edu
> <http://genome-mysql.cse.ucsc.edu>>
>
> db.user=genomep
> db.password=password
> central.db=hgcentral
>
> # command and error, called from directory containing
> phastCons46way.wib - why doesn't it use the local copy?
> hgWiggle -db=hg19 -chr=chr22 phastCons46way
> No such file or directory
> mustOpenFd: Can't open
> /gbdb/hg19/multiz46way/____phastCons46way.wib to read
>
>
>
>
>
> On Mon 30 Jun 2014 04:30:35 PM EDT, Luvina Guruvadoo
> wrote:
>
> Hello Kipper,
>
> Thank you for your question. In order to run
> hgWiggle, you
> will need
> to download the .wib file into a local directory.
> Please
> refer to our
> instructions here:
> http://genomewiki.ucsc.edu/____index.php/Using_hgWiggle_____without_a_database
> <http://genomewiki.ucsc.edu/__index.php/Using_hgWiggle___without_a_database>
>
> <http://genomewiki.ucsc.edu/__index.php/Using_hgWiggle___without_a_database
> <http://genomewiki.ucsc.edu/index.php/Using_hgWiggle_without_a_database>>.
>
>
> If you have any further questions, please reply to
> gen...@soe.ucsc.edu <mailto:gen...@soe.ucsc.edu>
> <mailto:gen...@soe.ucsc.edu <mailto:gen...@soe.ucsc.edu>>
> <mailto:gen...@soe.ucsc.edu
> <mailto:gen...@soe.ucsc.edu> <mailto:gen...@soe.ucsc.edu
> <mailto:gen...@soe.ucsc.edu>>>.
>
> All messages sent to that address are
> archived on a publicly-accessible forum. If your
> question
> includes
> sensitive data, you may send it instead to
> genom...@soe.ucsc.edu <mailto:genom...@soe.ucsc.edu>
> <mailto:genom...@soe.ucsc.__edu
> <mailto:genom...@soe.ucsc.edu>>
> <mailto:genom...@soe.ucsc.
> <mailto:genom...@soe.ucsc.>____edu
>
> <mailto:genom...@soe.ucsc.__edu
> <mailto:genom...@soe.ucsc.edu>>>.
>
> - - -
> Luvina Guruvadoo
> UCSC Genome Bioinformatics Group
>
>
>
> On Sat, Jun 28, 2014 at 2:43 PM, Kipper Fletez-Brant
> <caflet...@gmail.com
> <mailto:caflet...@gmail.com>
> <mailto:cafletezbrant@gmail.__com
> <mailto:caflet...@gmail.com>>
> <mailto:cafletezbrant@gmail.
> <mailto:cafletezbrant@gmail.>____com
>
> <mailto:cafletezbrant@gmail.__com
> /gbdb/hg19/multiz46way/______phastCons46way.wib
>
> to read
>
> # attempt 2: use a sample text file
> userApps/bin/hgWiggle -doStats
> -bedFile=hgwiggleTest.txt -db=hg19
> phastCons46way -doAscii
> No such file or directory
> mustOpenFd: Can't open
> /gbdb/hg19/multiz46way/______phastCons46way.wib
>
> to read
>
> # attempt 3: just write whatever to screen
> userApps/bin/hgWiggle -db=hg19
> -position=chr1:28125-28872 <tel:28125-28872>
> <tel:28125-28872 <tel:28125-28872>>
> <tel:28125-28872 <tel:28125-28872>
> <tel:28125-28872 <tel:28125-28872>>> -rawDataOut
>
> -doStats phastCons46way
> No such file or directory
> mustOpenFd: Can't open

Matthew Speir

unread,
Jul 18, 2014, 5:37:32 PM7/18/14
to Kipper Fletez-Brant, Brian Lee, gen...@soe.ucsc.edu, Luvina Guruvadoo
Hi Kipper,

Thank you for your question about using hgWiggle. We have been unable to reproduce the error on our end. Are you still experiencing issues using hgWiggle with a local wib file?

If you have any further questions, please reply to gen...@soe.ucsc.edu. All messages sent to that address are archived on a publicly-accessible Google Groups forum. If your question includes sensitive data, you may send it instead to genom...@soe.ucsc.edu.

Matthew Speir
UCSC Genome Bioinformatics Group
        <caflet...@gmail.com <mailto:caflet...@gmail.com>
        <mailto:cafletezbrant@gmail.__com
        <mailto:caflet...@gmail.com>
        <mailto:cafletezbrant@gmail.__com
        <mailto:caflet...@gmail.com>>
                    <mailto:cafletezbrant@gmail.
        <mailto:cafletezbrant@gmail.>____com

                    <mailto:cafletezbrant@gmail.__com
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